Journal: bioRxiv

Article Title: Constitutive NF-κB Activation is Amplified by VSV in Aggressive PC3 Prostate Cancer Cells that Resist Viral Oncolysis

doi: 10.1101/2025.09.23.678061

Figure Lengend Snippet: PC3 and LNCaP cells were infected with two VSV strains: WT and the R1 mutant strain, at an MOI of 0.1 and 1. Cell death was then measured at 24, 48 and 72 hours using a PrestoBlue assay. Statistical analysis was performed on three rounds of experiments, each with three biological replicates for each treatment/timepoint. Log2 Fold Change (LFC) was calculated relative to mock treated cells at each of the timepoints and treatments.

Article Snippet: The LNCaP and PC3 human PrCa cell lines were obtained from the American Type Culture Collection (ATCC) (CRL-1740 and CRL-1435, respectively) and were grown in aRPMI-1640 containing 10% FBS (ATCC).

Techniques: Infection, Mutagenesis, Prestoblue Assay

Journal: bioRxiv

Article Title: Constitutive NF-κB Activation is Amplified by VSV in Aggressive PC3 Prostate Cancer Cells that Resist Viral Oncolysis

doi: 10.1101/2025.09.23.678061

Figure Lengend Snippet: A) Immunofluorescence images of NF-κB localization in PC3 and LNCaP cells, following treatment with TNF- α , mock treatment or infection with VSV (WT and R1) at an MOI of 50 (PC3 cells) or 10 (LNCaP cells). B) NF-κB nuclear localization was quantitated by automatic counting of corrected total cell fluorescence (CTCF) in ImageJ. Statistical analysis was performed on three rounds of experiments, each performed using five biological replicates for each treatment. 10 images for each biological replicate were loaded into the software for counting. Statistical analysis was performed in R using a paired student’s t-test to compare PC3 to LNCaP cells in each treatment category (p < 0.001: ***; p < 0.01: **; p < 0.05: *).

Article Snippet: The LNCaP and PC3 human PrCa cell lines were obtained from the American Type Culture Collection (ATCC) (CRL-1740 and CRL-1435, respectively) and were grown in aRPMI-1640 containing 10% FBS (ATCC).

Techniques: Immunofluorescence, Infection, Fluorescence, Software

Journal: bioRxiv

Article Title: Constitutive NF-κB Activation is Amplified by VSV in Aggressive PC3 Prostate Cancer Cells that Resist Viral Oncolysis

doi: 10.1101/2025.09.23.678061

Figure Lengend Snippet: A LUMIT assay was performed to measure total and phosphorylated levels of IκB- α in PC3 and LNCaP cells (normalized to cell count), following mock infection or infection with VSV (WT and R1) at an MOI of 50 (PC3 cells) or 10 (LNCaP cells); samples were analyzed at the indicated time points (hours post-infection, hpi). Statistical analysis was performed on three rounds of experiments, with three biological replicates for each treatment. The Figure was generated in R and error bars were integrated by calculating the standard error of means.

Article Snippet: The LNCaP and PC3 human PrCa cell lines were obtained from the American Type Culture Collection (ATCC) (CRL-1740 and CRL-1435, respectively) and were grown in aRPMI-1640 containing 10% FBS (ATCC).

Techniques: Cell Counting, Infection, Generated

Journal: bioRxiv

Article Title: Constitutive NF-κB Activation is Amplified by VSV in Aggressive PC3 Prostate Cancer Cells that Resist Viral Oncolysis

doi: 10.1101/2025.09.23.678061

Figure Lengend Snippet: A LUMIT assay was performed to measure total and phosphorylated levels of p65 in PC3 and LNCaP cells (normalized to cell count), following mock infection or infection with VSV (WT and R1) at an MOI of 50 (PC3 cells) or 10 (LNCaP cells); samples were analyzed at the indicated time points (hours post-infection, hpi). Statistical analysis was performed on three rounds of experiments, with three biological replicates for each treatment. The Figure was generated in R and error bars were integrated by calculating the standard error of means.

Article Snippet: The LNCaP and PC3 human PrCa cell lines were obtained from the American Type Culture Collection (ATCC) (CRL-1740 and CRL-1435, respectively) and were grown in aRPMI-1640 containing 10% FBS (ATCC).

Techniques: Cell Counting, Infection, Generated

Journal: bioRxiv

Article Title: Constitutive NF-κB Activation is Amplified by VSV in Aggressive PC3 Prostate Cancer Cells that Resist Viral Oncolysis

doi: 10.1101/2025.09.23.678061

Figure Lengend Snippet: mRNA expression levels for selected antiviral/proinflammatory genes were measured in PC3 and LNCaP cells, following mock infection or infection with VSV (WT and R1) at an MOI of 50 (PC3 cells) or 10 (LNCaP cells); samples were analyzed at 6 and 8 hpi for PC3 and LNCaP, respectively. Sequencing analysis was performed on three biological replicates for each treatment. Statistical analysis was performed in R using a paired student’s t-test comparing PC3 to LNCaP cells in each treatment category and error bars were integrated by calculating the standard error of means (p.value < 0.001: “***”, p.value < 0.01: “**”, p.value < 0.05: “*”).

Article Snippet: The LNCaP and PC3 human PrCa cell lines were obtained from the American Type Culture Collection (ATCC) (CRL-1740 and CRL-1435, respectively) and were grown in aRPMI-1640 containing 10% FBS (ATCC).

Techniques: Expressing, Infection, Sequencing

Journal: bioRxiv

Article Title: Constitutive NF-κB Activation is Amplified by VSV in Aggressive PC3 Prostate Cancer Cells that Resist Viral Oncolysis

doi: 10.1101/2025.09.23.678061

Figure Lengend Snippet: Cell survival levels, with and without the NF-κB pathway inhibitor Bay11-7082, were measured in PC3 cells, following DMSO control treatment or infection with VSV (WT and R1) at an MOI of 50. Samples were run in duplicates and analyzed with PrestoBlue assay at 24, 48, and 72h post-treatment with Bay11-7082. Viral infection was initiated 8 hours prior to analysis at each timepoint. Statistical analysis was performed in R using student’s t-test comparing each treatment category and error bars were integrated by calculating the standard error of means (p.value < 0.0001: “****”, p.value < 0.001: “***”, p.value < 0.01: “**”, p.value < 0.05: “*”).

Article Snippet: The LNCaP and PC3 human PrCa cell lines were obtained from the American Type Culture Collection (ATCC) (CRL-1740 and CRL-1435, respectively) and were grown in aRPMI-1640 containing 10% FBS (ATCC).

Techniques: Control, Infection, Prestoblue Assay

Journal: Radiation Oncology (London, England)

Article Title: Resveratrol enhances prostate cancer cell response to ionizing radiation. Modulation of the AMPK, Akt and mTOR pathways

doi: 10.1186/1748-717X-6-144

Figure Lengend Snippet: Effects of resveratrol (RSV) and ionizing radiation (IR) on survival of prostate cancer (PrCa) cells . A . Cells were incubated with increasing doses of RSV (0-10 μM) and allowed to form colonies. Colonies (> 50 cells) were counted. Results are expressed as the surviving fraction compared to untreated control (see Methods) . '*' p < 0.05 compared to untreated PC3 cells, '#' p < 0.05 compared to untreated 22RV1 cells, '+' p < 0.05 compared to untreated PNT1A cells. B . Cells were pre-treated with RSV (0-10 μM) followed by treatment with a single fraction of IR (2 Gy) and allowed to grow and form colonies. Treatment values were normalized to untreated controls and surviving fractions are presented as percent of control. Shown is the mean of 4-5 independent experiments ± standard error (SE). '*' p < 0.05 compared to 2 Gy treated PC3 cells, '#' p < 0.05 compared to 2 Gy treated 22RV1 cells, '+' p < 0.05 compared to 2 Gy treated PNT1A cells. C . Projected proportion of tumour survival after treatment with 0 - 80 Gy was estimated using the Surviving Fraction at 2 Gy (SF2) values for control and RSV (5 μM) treated PNT1A, PC3 and 22RV1 cells. SF2 n was determined as the tumour proportion surviving after "n" 2 Gy fractions, assuming iso-effectiveness of each fraction. Values were plotted up to a scale of 1 × 10 -11 to reflect elimination of tumour of 50 - 100 gr expected to contain 5 - 10 × 10 10 cells.

Article Snippet: Human PrCa (PC3, 22RV1) and normal prostate epithelial (PNT1A) cell lines were obtained from American Tissue Culture Collection (Manassas, VA, U.S.A.).

Techniques: Incubation, Control